NLS (computer system), a 1960s computing collaboration
National Language Support (or Native Language Support), in HP software
Organisations
Government
National Language Services, South Africa
National Longitudinal Surveys, U.S.
National Lifeguard Service, Canada
Businesses
Non-Linear Systems, electronics manufacturer
Nautilus, Inc., stock symbol NLS, fitness products manufacturer
Education
North Leamington School, England
Nottingham Law School, England
National Law School of India University, Bangalore
Librarial
National Library Service for the Blind and Print Disabled, American mail service
National Library of Scotland
National Life Stories, at the British Library
Political
Labour Students, formally called National Labour Students
Science and technology
Nuclear localization signal, in biology
Nonlinear Schrödinger equation, in physics
Non-linear least squares, in statistics, a method used in regression analysis
Spaceflight
Nanosatellite Launch System, based at the University of Toronto
National Launch System, a 1991 Space Shuttle alternative study
Transport
Nailsea and Backwell railway station station code NLS, England
Niles (Amtrak station) station code NLS, Michigan, United States
Other
Nimrod Line Squadron, former military service unit at RAF Kinloss
National League System, in English football
Nürburgring Langstrecken Serie, a motorsport endurance series held at the Nürburgring. Formerly known as VLN (Veranstaltergemeinschaft Langstreckenpokal Nürburgring)
New Face of Serbia (Novo lice Srbije), a political party in Serbia